7.4.4 Cellulase Enzyme Solution (Cellulase) 7.4.4.1 Immediately before use, prepare a solution containing 2-6 units/ml of Cellulase in cold deionized water. 12 PrepMan Ultra Sample Preparation Reagent Protocol About the PrepMan Ultra Sample Preparation Reagent Purpose of PrepMan Ultra Sample Preparation Reagent PrepMan® Ultra Sample Preparation Reagent provides a simple way to prepare DNA from a wide range of sample types including: † Processed foods and their ingredients † Bacteria † Fungi Add to a small amount of cold water in a beaker and make a slurry. First, take the absorbance (OD) of Blank and make it zero. 4H 2 O) Add 20cm 3 of 2N NaOH. DNS method The DNS method for estimating the concentration of reducing sugars in a sample Reducing sugars contain free carbonyl group, have the property to reduce many of the reagents. The DNSA test can detect concentrations of glucose between 0.5 mM (0.09% glucose w/v) and 40 mM (0.72% glucose w/v). Keep in boiling water bath for 15 minutes. Dilute to a final volume of 100cm 3 with water. 7.4.3 Glucose (HK) Determination Vial. Mix and heat gently to make a uniform suspension. Use a good quality starch, e.g. PREPARATION. Thamara C. Coutinho, João O. D. Malafatti, Elaine C. Paris, Paulo W. Tardioli, Cristiane S. Farinas. necessary. The colour of the reagent changes from yellow to orange or red, depending upon the concentration of reducing sugar present. Take 7 clean, dry test tubes. Generate a calibration curve to correlate the absorbance to the sucrose concentration. This starch solution does not keep well and should be made up fresh on the required day. Heat the mixture at 90º C for 5-15 minutes to develop the red-brown color. (To avoid the loss of liquid due to evaporation, cover the test tube with a piece of paraffin film if a plain test tube is used.) Analar. Weigh out 2 g starch powder. Discussions The DNS method can be applied twice to measure the individual concentrations of a mixture of glucose and sucrose. Dissolve contents per label instructions. Mix well. No. The DNSA reagent base is supplied without sodium hydroxide. This stock solution is stable for at least 2 weeks at room temperature. Pipette out standard sugar solution in the range of 0 to 3 mL in different test tubes and make up the volume of all test tubes to 3 mL with distilled water concentrations ranging from 0 to 750 mg. Add 1 mL DNS reagent to all the test tubes and mix plug the test tube with cotton or marble and keep the test tube in a boiling water bath for 5 minute. After cooling to room temperature in a cold water bath, record the absorbance with a spectrophotometer at 540nm. Add the DNS reagent and follow the DNS method henceforth. Then make up to 100 cm3 with boiling water, stirring constantly. Prepare arsenomolybdate reagent in three steps: Dissolve 25 g ammonium molybdate in 400 mL water and add 25 mL concentrated sulfuric acid and mix. 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